4CSC

STRUCTURE OF TERNARY COMPLEXES OF CITRATE SYNTHASE WITH D-AND L-MALATE: MECHANISTIC IMPLICATIONS

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	104.000, 78.100, 58.300
Unit cell angles	90.00, 78.90, 90.00

Refinement procedure

Resolution	6.000 - 1.900
R-factor	0.188
RMSD bond length	0.024
RMSD bond angle	2.500
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.097 *
Total number of observations	51703 *
Number of reflections	24109 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *		referred to 3cts *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.0 (mg/ml)
2	1	drop	sodium citrate	0.9 (%)
3	1	drop	CoA	1 (mM)
4	1	reservoir	sodium citrate	1.2 (M)