4CS9
Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer bound to adenosine monophosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-12 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.050, 93.610, 85.600 |
Unit cell angles | 90.00, 95.76, 90.00 |
Refinement procedure
Resolution | 49.800 - 2.010 |
R-factor | 0.1875 |
Rwork | 0.186 |
R-free | 0.20830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4cs8 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.960 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.800 | 2.060 |
High resolution limit [Å] | 2.010 | 2.010 |
Rmerge | 0.070 | 0.840 |
Number of reflections | 51659 | |
<I/σ(I)> | 22.5 | 1.8 |
Completeness [%] | 98.8 | 85.1 |
Redundancy | 12.8 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 28 % W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 2000, 0.100 M BIS-TRIS PH 6.5 |