4CS5
Crystal Structure of PCNA from Litopenaeus vannamei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-31 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.570, 83.380, 74.310 |
| Unit cell angles | 90.00, 117.65, 90.00 |
Refinement procedure
| Resolution | 28.058 - 3.000 |
| R-factor | 0.2672 |
| Rwork | 0.265 |
| R-free | 0.31080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HOMOLOGY MODEL OF LVPCNA BASED ON PDB ENTRY 1VYM |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.489 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.420 |
| Number of reflections | 14802 | |
| <I/σ(I)> | 10.69 | 3.57 |
| Completeness [%] | 93.3 | 97 |
| Redundancy | 2.96 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 300 MM CACL2, 100 MM SODIUM HEPES PH 7.5 AND 30%(V/V) PEG 400 |
