4CRY
Direct visualisation of strain-induced protein post-translational modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2013-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 |
| Unit cell lengths | 86.400, 86.400, 81.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.190 - 1.610 |
| R-factor | 0.12972 |
| Rwork | 0.129 |
| R-free | 0.15017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4azd |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.704 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.100 | 1.640 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.060 | 0.500 |
| Number of reflections | 37491 | |
| <I/σ(I)> | 11.8 | 1.6 |
| Completeness [%] | 97.4 | 80.1 |
| Redundancy | 4.1 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 20% (W/V) POLYETHYLENE GLYCOL (PEG) 3350, 0.1 M BIS-TRIS PROPANE PH 7.4, 0.2 M POTASSIUM THIOCYANATE |
