4CRW

Complex of human DDX6 (RECA-C) and CNOT1 (MIF4G)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-10-27
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	43.900, 90.840, 95.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.885 - 1.750
R-factor	0.1636
Rwork	0.162
R-free	0.20300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 2WAX (CHAIN A) 4GML (CHAIN A)
RMSD bond length	0.011
RMSD bond angle	1.306
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.900	1.800
High resolution limit [Å]	1.750	1.750
Number of reflections	39120
<I/σ(I)>	17.1	2.3
Completeness [%]	99.2	99.5
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		0.1M TRIS-CL PH=8, 16% PEG6000,, pH 8.0