4CRQ
Crystal structure of the catalytic domain of the modular laminarinase ZgLamC mutant E142S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.108, 68.061, 143.282 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.980 - 1.500 |
| R-factor | 0.14987 |
| Rwork | 0.148 |
| R-free | 0.18576 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iln |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.686 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.340 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.050 | 0.690 |
| Number of reflections | 105701 | |
| <I/σ(I)> | 17.9 | 2.6 |
| Completeness [%] | 99.8 | 98.5 |
| Redundancy | 6.6 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 14% PEG 6000, 100 MM NA ACETATE PH 5.0, 220 MM MGCL2 AND 10% GLYCEROL. |
