4CRO
PROTEIN-DNA CONFORMATIONAL CHANGES IN THE CRYSTAL STRUCTURE OF A LAMBDA CRO-OPERATOR COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | rotating-anode X-ray tube |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | AREA DETECTOR |
| Spacegroup name | P 32 |
| Unit cell lengths | 154.800, 154.800, 86.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 3.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.9 | referred to J.Mol.Biol. 188.115-118 1986 * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | NA CACODYLATE | ||
| 3 | 1 | 1 | NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | cro monomer | 2.5 (mg/ml) | |
| 2 | 1 | drop | duplex 17 base-pair fragment | 2.5 (mg/ml) | |
| 3 | 1 | drop | sodium cacodylate | 20 (mM) | |
| 4 | 1 | drop | 100 (mM) | ||
| 5 | 1 | reservoir | 3.5-4.0 (M) |
