4CR0

Crystal Structure of H5 (VN1194) Asn186Lys/Gly143Arg Mutant Haemagglutinin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 63
Unit cell lengths	77.950, 77.950, 210.411
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	105.210 - 2.650
R-factor	0.22651
Rwork	0.225
R-free	0.26200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bgw
RMSD bond length	0.007
RMSD bond angle	1.163
Data reduction software	xia2
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0046)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.510	2.790
High resolution limit [Å]	2.650	2.650
Rmerge	0.140	0.550
Number of reflections	20820
<I/σ(I)>	9.8	2.6
Completeness [%]	99.1	99.1
Redundancy	5.5	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M MOPS PH 7.0, 12% PEG 4000