4CQI

Crystal structure of recombinant tubulin-binding cofactor A (TBCA) from Leishmania major

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-17
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 31 2 1
Unit cell lengths	76.757, 76.757, 39.433
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.430 - 1.900
R-factor	0.18713
Rwork	0.185
R-free	0.22760
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.013
RMSD bond angle	1.586
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.400	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.450
Number of reflections	10821
<I/σ(I)>	24.9	6.8
Completeness [%]	100.0	100
Redundancy	12.1	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				RESERVOIR CONTAINED 0.2M (NH4)2HPO4 AND 1.6M (NH4)2SO4.