4CPF
Wild-type streptavidin in complex with love-hate ligand 3 (LH3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 47.080, 94.120, 104.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.748 - 1.140 |
| R-factor | 0.1401 |
| Rwork | 0.139 |
| R-free | 0.16460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ry1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.393 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.750 | 1.180 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.050 | 0.340 |
| Number of reflections | 79019 | |
| <I/σ(I)> | 18.8 | 2.83 |
| Completeness [%] | 93.0 | 65 |
| Redundancy | 6.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 75% SATURATED AMMONIUM SULPHATE, 25% 1M SODIUM ACETATE PH4.5. SITTING DROP |
