4CP6
The Crystal structure of Pneumococcal vaccine antigen PcpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-23 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.060, 61.850, 121.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.200 - 1.340 |
| Rwork | 0.170 |
| R-free | 0.18600 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.140 |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.200 | 1.360 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rmerge | 0.060 | 0.740 |
| Number of reflections | 103647 | |
| <I/σ(I)> | 18.1 | 1.7 |
| Completeness [%] | 98.5 | 85.2 |
| Redundancy | 6.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | pH 4.6 |
