4CP6
The Crystal structure of Pneumococcal vaccine antigen PcpA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-23 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.060, 61.850, 121.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.200 - 1.340 |
Rwork | 0.170 |
R-free | 0.18600 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.140 |
Phasing software | SHARP |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.200 | 1.360 |
High resolution limit [Å] | 1.340 | 1.340 |
Rmerge | 0.060 | 0.740 |
Number of reflections | 103647 | |
<I/σ(I)> | 18.1 | 1.7 |
Completeness [%] | 98.5 | 85.2 |
Redundancy | 6.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | pH 4.6 |