4CP2
Crystal Structure of Epithelial Adhesin 9 A domain (Epa9A) from Candida glabrata in complex with Galb1-4GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.840, 66.840, 115.950 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.000 - 2.600 |
| R-factor | 0.18392 |
| Rwork | 0.181 |
| R-free | 0.24228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PRUNED VERSION OF PDB ENTRY 4AF9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.312 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.960 | 2.720 |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.120 | 0.580 |
| Number of reflections | 9889 | |
| <I/σ(I)> | 15.7 | 3.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.1 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 0.1 M MAGNESIUM CHLORIDE, 0.1M SODIUM CHLORIDE, 0.1 M MES PH6.5, 12% PEG 4000, 0.05 M LACTOSE, VAPOR DIFFUSION IN SITTING DROP, SOAKED WITH GALB1-4GLCNAC |
