4CP1
Crystal Structure of Epithelial Adhesin 9 A domain (Epa9A) from Candida glabrata in complex with Galb1-3GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-16 |
| Detector | MARRESEARCH MARMOSAIC 255 MM |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.660, 66.660, 117.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.350 - 2.500 |
| R-factor | 0.17279 |
| Rwork | 0.170 |
| R-free | 0.23056 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PRUNED VERSION OF PDB ENTRY 4AF9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.487 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.210 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.140 | 0.510 |
| Number of reflections | 10922 | |
| <I/σ(I)> | 12.5 | 3.4 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 4.8 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 0.1 M HEPES PH7, 32% PEG 6000, 0.05 M LACTOSE, VAPOR DIFFUSION IN SITTING DROP, SOAKED WITH GALB1-3GLCNAC |
