4COW
Crystal Structure of Epithelial Adhesin 6 A domain (Epa6A) from Candida glabrata in complex with the T-antigen (Galb1-3GalNAc)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.350, 61.100, 106.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.690 - 2.150 |
R-factor | 0.17963 |
Rwork | 0.177 |
R-free | 0.22973 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4af9 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.323 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.240 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.120 | 0.600 |
Number of reflections | 18879 | |
<I/σ(I)> | 11.9 | 2.7 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 5.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.08 M SODIUM ACETATE PH:4.6, 1.6 M AMMONIUM SULFATE, 20% GLYCEROL, 0.05 M LACTOSE, 291 K, VAPOR DIFFUSION IN SITTING DROP, SOAKED WITH GALB1-3GALNAC |