4COU
Crystal Structure of Epithelial Adhesin 6 A domain (Epa6A) from Candida glabrata in complex with Lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-16 |
| Detector | MARRESEARCH MARMOSAIC 255 MM |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.300, 61.100, 106.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.500 - 1.480 |
| R-factor | 0.145 |
| Rwork | 0.144 |
| R-free | 0.17152 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PRUNED VERSION OF PDB ENTRY 4AF9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.268 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.770 | 1.560 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.040 | 0.570 |
| Number of reflections | 45573 | |
| <I/σ(I)> | 21.1 | 2.9 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.08 M SODIUM ACETATE PH:4.6, 1.6 M AMMONIUM SULFATE, 20% GLYCEROL, 0.05 M LACTOSE, 291 K, VAPOR DIFFUSION IN SITTING DROP |
