4COS
Crystal structure of the PHD-Bromo-PWWP cassette of human PRKCBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.955, 68.835, 70.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.200 - 1.670 |
| R-factor | 0.17753 |
| Rwork | 0.176 |
| R-free | 0.21039 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.712 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 1.770 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.080 | 0.770 |
| Number of reflections | 38079 | |
| <I/σ(I)> | 12.2 | 2 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.3 | pH 6.3 |
