4COP
HIV-1 capsid C-terminal domain mutant (Y169S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.560, 72.390, 35.700 |
| Unit cell angles | 90.00, 109.32, 90.00 |
Refinement procedure
| Resolution | 10.210 - 1.850 |
| R-factor | 0.2361 |
| Rwork | 0.236 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2buo |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.040 | 0.500 |
| Number of reflections | 12273 | |
| <I/σ(I)> | 26.1 | 3.8 |
| Completeness [%] | 91.4 | 61.2 |
| Redundancy | 3.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10.5 | 1.2 M NAH2PO4, 0.8 M K2HPO4, 0.1 M CAPS PH 10.5 AND 0.2 M LISO4 |
