4COC
HIV-1 capsid C-terminal domain mutant (Y169L)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.480, 33.760, 77.730 |
| Unit cell angles | 90.00, 108.32, 90.00 |
Refinement procedure
| Resolution | 47.420 - 1.590 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.22323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2buo |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.642 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.420 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.090 | 0.520 |
| Number of reflections | 33062 | |
| <I/σ(I)> | 13.1 | 2 |
| Completeness [%] | 97.9 | 77.8 |
| Redundancy | 5.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 30% PEG 4000, 0.2 M LISO4, 0.1 M TRIS PH 8.5 |
