4COB
Crystal structure kynurenine formamidase from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 112.721, 112.721, 90.761 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 97.620 - 2.370 |
| R-factor | 0.15265 |
| Rwork | 0.150 |
| R-free | 0.19367 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r61 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.893 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Rmerge | 0.060 | 0.570 |
| Number of reflections | 27365 | |
| <I/σ(I)> | 16.8 | 2.4 |
| Completeness [%] | 99.2 | 94.5 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 0.1 M HEPES PH 7.5, 20 % (W/V) PEG [POLY(ETHYLENE GLYCOL)] 4000, AND 10 % (V/V) 2-PROPANOL. 293 K. |
