4CO2

Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine diphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-12-06
Detector	DECTRIS PILATUS 6M
Spacegroup name	H 3
Unit cell lengths	89.350, 89.350, 67.120
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.520 - 1.800
R-factor	0.1781
Rwork	0.176
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cny
RMSD bond length	0.007
RMSD bond angle	1.289
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.520	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.100	0.750
Number of reflections	18406
<I/σ(I)>	13.9	2.3
Completeness [%]	99.4	100
Redundancy	5.72	5.74

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	10 MM MGCL2, 5 MM ATP, 0.1 M TRIS-HCL PH 8.5, 30% PEG 300, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K