4CO1
Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 |
| Unit cell lengths | 89.430, 89.430, 67.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.890 - 1.500 |
| R-factor | 0.1463 |
| Rwork | 0.139 |
| R-free | 0.17120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cny |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.100 | 0.300 |
| Number of reflections | 32015 | |
| <I/σ(I)> | 12.1 | 5.3 |
| Completeness [%] | 88.5 | 99.8 |
| Redundancy | 5.32 | 4.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10 MM ADP, 0.1 M NA HEPES PH 7.5, 25% PEG 1000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
