4CNZ
Structure of PII signaling protein GlnZ from Azospirillum brasilense in complex with adenosine diphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-05-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.324, 87.350, 119.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.815 - 1.700 |
| R-factor | 0.1941 |
| Rwork | 0.192 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cny |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.254 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.800 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.110 | 0.700 |
| Number of reflections | 74797 | |
| <I/σ(I)> | 11.1 | 2.3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.95 | 6.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0 MM ADP, 0.02 M CACL, 0.1 M NAAC PH 4.6, 30% MPD, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
