4CNQ
Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.870, 68.950, 156.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.550 - 1.840 |
| R-factor | 0.17511 |
| Rwork | 0.172 |
| R-free | 0.22735 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yml |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.330 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.550 | 8.250 |
| High resolution limit [Å] | 1.890 | 1.840 |
| Rmerge | 0.070 | 0.770 |
| Number of reflections | 259162 | |
| <I/σ(I)> | 18.4 | 2.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | pH 4.5 |
