4CNL
Crystal structure of the Choline-binding domain of CbpL from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.855, 42.789, 70.623 |
| Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
| Resolution | 36.410 - 1.700 |
| R-factor | 0.1534 |
| Rwork | 0.151 |
| R-free | 0.19020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.790 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.130 |
| Number of reflections | 19551 | |
| <I/σ(I)> | 27.5 | 11.1 |
| Completeness [%] | 97.7 | 96.8 |
| Redundancy | 6.7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6 M AMMONIUM SULFATE, 0.5 M LICL |
