4CNJ
L-Aminoacetone oxidase from Streptococcus oligofermentans belongs to a new 3-domain family of bacterial flavoproteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 79.149, 130.346, 224.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.140 - 2.700 |
| R-factor | 0.19063 |
| Rwork | 0.188 |
| R-free | 0.23852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gqf |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.342 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.680 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.100 | 0.530 |
| Number of reflections | 68683 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 98.7 | 99.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 2.0 M (NH4)2SO4, 0.1 M NA-CACODYLATE OR MES (PH 6.5), AND 0.2 M NACL |
