4CN8
Structure of proximal thread matrix protein 1 (PTMP1) from the mussel byssus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.530, 44.540, 85.850 |
| Unit cell angles | 90.00, 105.93, 90.00 |
Refinement procedure
| Resolution | 30.832 - 2.450 |
| R-factor | 0.2068 |
| Rwork | 0.205 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1AOX 1qc5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MrBUMP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 2.500 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.100 | 0.770 |
| Number of reflections | 15438 | |
| <I/σ(I)> | 13.3 | 1.7 |
| Completeness [%] | 97.0 | 74.6 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 30% PEG2000-MME, 0.2 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.6 |
