4CN4
GlgE isoform 1 from Streptomyces coelicolor E423A mutant with 2-deoxy- 2-fluoro-beta-maltosyl modification
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 113.000, 113.000, 312.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.540 - 2.400 |
| R-factor | 0.18525 |
| Rwork | 0.184 |
| R-free | 0.20848 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zss |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.540 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.990 |
| Number of reflections | 79517 | |
| <I/σ(I)> | 14.7 | 2 |
| Completeness [%] | 99.5 | 95.3 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
