4CL2
structure of periplasmic metal binding protein from candidatus liberibacter asiaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-09-07 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.060, 94.060, 94.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.290 - 1.630 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hh8 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.797 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.850 | 1.720 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.070 | 0.920 |
| Number of reflections | 57802 | |
| <I/σ(I)> | 18.5 | 2.1 |
| Completeness [%] | 95.7 | 92.1 |
| Redundancy | 8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1M SODIUM ACETATE TRIHYDRATE PH 4.6, 2M AMMONIUM SULPHATE |
