4CKN
Structure of an N-terminal fragment of Leishmania SAS-6 containing parts of its coiled coil domain, F257E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.855, 81.253, 133.149 |
| Unit cell angles | 90.00, 91.50, 90.00 |
Refinement procedure
| Resolution | 133.100 - 2.900 |
| R-factor | 0.23775 |
| Rwork | 0.237 |
| R-free | 0.25597 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ckm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.255 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.910 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.150 | |
| Number of reflections | 25904 | |
| <I/σ(I)> | 10.6 | 0.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.85 | 100 MM NACITRATE PH 5.85, 21% (W/V) PEG-3000 |
