4CKB
Vaccinia virus capping enzyme complexed with GTP and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.340, 197.730, 61.110 |
| Unit cell angles | 90.13, 109.27, 94.05 |
Refinement procedure
| Resolution | 49.200 - 2.800 |
| R-factor | 0.21118 |
| Rwork | 0.209 |
| R-free | 0.25477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vdw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.126 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.650 |
| Number of reflections | 62966 | |
| <I/σ(I)> | 10.75 | 1.43 |
| Completeness [%] | 96.1 | 90.3 |
| Redundancy | 2.37 | 2.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PROTEIN SOLUTION AT 4 MG/ML IN 40 MM TRIS-HCL, PH 8.0, 200 MM NACL, 5 MM DTT WITH 100 MM GTP, 5 MM MANGANESE CHLORIDE, AND 5 MM ADOMET. RESERVOIR SOLUTION 0.1 M CITRIC ACID, PH 6.5, 1.2 M LICL, 6% PEG6000 |
