4CK4
Ovine beta-Lactoglobulin at Atomic Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX14.2 |
| Synchrotron site | SRS |
| Beamline | PX14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.148, 49.031, 49.053 |
| Unit cell angles | 69.75, 69.08, 77.56 |
Refinement procedure
| Resolution | 14.700 - 1.120 |
| R-factor | 0.14078 |
| Rwork | 0.139 |
| R-free | 0.16942 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gxa |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.926 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.140 |
| High resolution limit [Å] | 1.120 | 1.120 |
| Rmerge | 0.050 | 1.230 |
| Number of reflections | 116059 | |
| <I/σ(I)> | 9.4 | 3.7 |
| Completeness [%] | 92.3 | 89.4 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 0.2M SODIUM CITRATE, 0.1M SODIUM POTASSIUM TARTRATE, 2.0M AMMONIUM SULPHATE, PH 5.6 |
