4CJO
Spectroscopically-validated structure of ferrous cytochrome c prime from Alcaligenes xylosoxidans, reduced at 180K using X-rays
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 180 |
| Detector technology | PIXEL |
| Collection date | 2013-03-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.675, 53.675, 181.812 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.480 - 1.550 |
| R-factor | 0.23222 |
| Rwork | 0.231 |
| R-free | 0.25119 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.065 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.060 | 0.660 |
| Number of reflections | 21722 | |
| <I/σ(I)> | 12.5 | 2.4 |
| Completeness [%] | 93.9 | 96 |
| Redundancy | 4.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULPHATE, HEPES PH 7.5 |
