4CJ7
Structure of Crenactin, an archeal actin-like protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-01 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 78.127, 78.127, 418.958 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.860 - 3.200 |
| R-factor | 0.23811 |
| Rwork | 0.235 |
| R-free | 0.29549 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yvn |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.975 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.310 | 1.500 |
| Number of reflections | 22716 | |
| <I/σ(I)> | 8.6 | 1.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 12.6 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M LITHIUM SULFATE, 0.1 M PHOSPHATE CITRATE PH 4.2, 20% W/V PEG 1000 |
