4CIH
Structure of LntA-K180D-K181D from Listeria monocytogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2012-08-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 |
| Unit cell lengths | 141.188, 141.188, 60.442 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.060 - 2.220 |
| R-factor | 0.28102 |
| Rwork | 0.278 |
| R-free | 0.30851 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xl4 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.023 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.290 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.010 | 0.610 |
| Number of reflections | 55609 | |
| <I/σ(I)> | 25.4 | 3 |
| Completeness [%] | 93.9 | 66.6 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1 M NA CITRATE PH 5.0 1M LICL2 21% PEG3350 |
