4CI6
Mechanisms of crippling actin-dependent phagocytosis by YopO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.799, 121.850, 89.339 |
| Unit cell angles | 90.00, 94.87, 90.00 |
Refinement procedure
| Resolution | 20.022 - 2.651 |
| R-factor | 0.1873 |
| Rwork | 0.186 |
| R-free | 0.22010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3HBT 2h7o |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.605 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 41770 | |
| <I/σ(I)> | 12.75 | 2.65 |
| Completeness [%] | 95.9 | 75.1 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG400, 0.1M HEPES PH7.5, 0.2M SODIUM CHLORIDE |
