4CHX
Crystal structure of MltC in complex with disaccharide pentapeptide DHl89
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.160, 115.000, 61.280 |
| Unit cell angles | 90.00, 93.21, 90.00 |
Refinement procedure
| Resolution | 45.916 - 2.450 |
| R-factor | 0.1963 |
| Rwork | 0.192 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c5f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.421 |
| Data scaling software | SCALA |
| Phasing software | iMOSFLM |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.080 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.180 | 0.930 |
| Number of reflections | 25551 | |
| <I/σ(I)> | 7.2 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 18% PEG 3350, 0.2M TRIAMMONIUM CITRATE PH 7.5 |
