4CHU
E. coli IscR-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.015, 75.835, 173.421 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.973 - 2.489 |
| R-factor | 0.209 |
| Rwork | 0.207 |
| R-free | 0.25080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | T. POTENS ISCR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.381 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.100 | 0.910 |
| Number of reflections | 23387 | |
| <I/σ(I)> | 8.6 | 1.5 |
| Completeness [%] | 99.3 | 96.3 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.1M CITRIC ACID PH 4.0, 0.1M LICL, 20% (W/V) PEG6K |
