4CHL
Human Ethylmalonic Encephalopathy Protein 1 (hETHE1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-17 |
| Detector | DECTRIS HYBRID |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.940, 124.930, 63.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.631 - 2.610 |
| R-factor | 0.1808 |
| Rwork | 0.178 |
| R-free | 0.22320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gcu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.742 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.940 | 2.680 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.120 | 0.890 |
| Number of reflections | 18351 | |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 99.7 | 99.2 |
| Redundancy | 6.5 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 10 MG/ML PROTEIN IN 50MM HEPES PH7.5, 100 MM NACL ADDED TO 0.1M TRIS-HCL PH8.5, 0.5M POTASSIUM THIOCYANATE |
