4CHH
N-terminal domain of yeast PIH1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-22 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.750, 48.290, 96.280 |
| Unit cell angles | 90.00, 98.10, 90.00 |
Refinement procedure
| Resolution | 47.660 - 2.030 |
| R-factor | 0.188 |
| Rwork | 0.185 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE MOUSE PIH |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.040 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.290 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 23130 | |
| <I/σ(I)> | 9.7 | 2.2 |
| Completeness [%] | 97.9 | 87.6 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
