4CHD
Crystal structure of the '627' domain of the PB2 subunit of Thogoto virus polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.480, 65.480, 74.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.270 - 2.400 |
| R-factor | 0.20891 |
| Rwork | 0.207 |
| R-free | 0.23904 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.560 |
| Number of reflections | 6604 | |
| <I/σ(I)> | 11.1 | 2.6 |
| Completeness [%] | 97.4 | 98.7 |
| Redundancy | 3.95 | 4.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | THE PROTEIN WAS CONCENTRATED TO 2.3 MG/ML IN 250 MM NACL, 50 MM TRIS PH 8.0 AND 2 MM DTT AND CRYSTALS WERE GROWN IN 0.1 M HEPES PH 7.0, 0.2 M AMMONIUM SULPHATE AND 22% PEG3350 |
