4CGX
Crystal structure of the N-terminal domain of the PA subunit of Thogoto virus polymerase (form 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.930, 77.230, 67.750 |
| Unit cell angles | 90.00, 123.27, 90.00 |
Refinement procedure
| Resolution | 41.815 - 2.700 |
| R-factor | 0.2035 |
| Rwork | 0.200 |
| R-free | 0.27970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cgs |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.822 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.080 | 0.680 |
| Number of reflections | 9879 | |
| <I/σ(I)> | 13.5 | 2.1 |
| Completeness [%] | 98.1 | 97.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1 M MGCL2, 0.1 M MES PH 6.5 AND 22% PEG 4000 |
