4CGW
Second TPR of Spaghetti (RPAP3) bound to HSP90 peptide SRMEEVD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-21 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.570, 96.510, 52.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.255 - 3.001 |
| R-factor | 0.2437 |
| Rwork | 0.241 |
| R-free | 0.29130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE STRUCTURE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.671 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.260 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 6373 | |
| <I/σ(I)> | 12.5 | 2 |
| Completeness [%] | 94.3 | 94.6 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7 |
