4CGP

Leishmania major N-myristoyltransferase in complex with cofactor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-12
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	47.711, 91.197, 53.019
Unit cell angles	90.00, 111.71, 90.00

Refinement procedure

Resolution	49.260 - 1.400
R-factor	0.16115
Rwork	0.159
R-free	0.19638
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.029
RMSD bond angle	2.670
Data reduction software	XDS
Data scaling software	SCALA
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.060	0.310
Number of reflections	80714
<I/σ(I)>	11.4	2.8
Completeness [%]	97.5	76.5
Redundancy	4	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		30% PEG 1500, 0.2 M NACL, 0.1 M NA CACODYLATE, PH 5.5