4CGB

Crystal structure of the trimerization domain of EML2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 21
Unit cell lengths	47.871, 51.135, 101.691
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.846 - 2.154
R-factor	0.2078
Rwork	0.205
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cgc
RMSD bond length	0.006
RMSD bond angle	0.930
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.860	2.320
High resolution limit [Å]	2.150	2.150
Rmerge	0.080	0.640
Number of reflections	14593
<I/σ(I)>	14.2	3.2
Completeness [%]	99.8	99.9
Redundancy	7	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5		CRYSTALS WERE OBTAINED BY THE HANGING DROP VAPOUR DIFFUSION METHOD USING 100 MM BIS-TRIS-PROPANE PH 6.5, 20% PEG 3350, 200 MM POTASSIUM THIOCYANATE IN THE RESERVOIR.