4CF4
Mutagenesis of a Rhodobacteraceae L-haloacid dehalogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 287 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.090, 86.640, 105.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.970 - 2.140 |
| R-factor | 0.20583 |
| Rwork | 0.202 |
| R-free | 0.28003 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yml |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.642 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.970 | 2.200 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 23818 | |
| <I/σ(I)> | 13.5 | 3.1 |
| Completeness [%] | 98.9 | 98.9 |
| Redundancy | 6.3 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 |
