4CEK
Crystal structure of the second MIF4G domain of human nonsense mediated decay factor UPF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.880, 56.600, 142.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.070 - 2.350 |
| R-factor | 0.2085 |
| Rwork | 0.206 |
| R-free | 0.25083 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.462 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD (SHARP) |
| Refinement software | REFMAC (5.6.0116) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.810 |
| Number of reflections | 14361 | |
| <I/σ(I)> | 13.8 | 1.9 |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.95 | 4.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM MES PH 6.0, 19% PEG 3350 AND AT A PROTEIN CONCENTRATION OF 8 MG/ML |
