4CDZ
Crystal Structure of Spinosyn Rhamnosyl 4'-O-Methyltransferase SpnH from Saccharopolyspora spinosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-31 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 54.773, 54.773, 307.937 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.835 - 2.503 |
| R-factor | 0.2145 |
| Rwork | 0.208 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wk1 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.840 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.110 | 0.670 |
| Number of reflections | 10187 | |
| <I/σ(I)> | 25.7 | 3.1 |
| Completeness [%] | 98.5 | 99.7 |
| Redundancy | 16.4 | 16.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 8% (W/V) POLYETHYLENE GLYCOL 8000, 100MM SODIUM CACODYLATE, PH6, 100MM MAGNESIUM ACETATE |
