4CDV
Spectroscopically-validated structure of cytochrome c prime from Alcaligenes xylosoxidans, reduced by X-ray irradiation at 100K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-14 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.281, 53.281, 180.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.140 - 1.170 |
| R-factor | 0.14061 |
| Rwork | 0.140 |
| R-free | 0.15998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.074 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.100 | 1.240 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.050 | 0.100 |
| Number of reflections | 49558 | |
| <I/σ(I)> | 17.1 | 2.6 |
| Completeness [%] | 95.0 | 69.7 |
| Redundancy | 5.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULFATE, HEPES PH 7.5 |
