Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-08 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 |
| Unit cell lengths | 106.487, 106.487, 90.229 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.080 - 1.450 |
| R-factor | 0.13462 |
| Rwork | 0.133 |
| R-free | 0.17253 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u9t |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.365 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.080 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.070 | |
| Number of reflections | 67643 | |
| <I/σ(I)> | 38 | 1.8 |
| Completeness [%] | 98.7 | 97.3 |
| Redundancy | 6.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277 | 20MG/ML PROTEIN, 12-15% PEG 0.15-0.2M MAGNESIUM FORMATE, AND 10-20MM NAD, PH 8.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 277K |
