4CDA
Spectroscopically-validated structure of ferric cytochrome c prime from Alcaligenes xylosoxidans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-28 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.354, 53.354, 180.992 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.210 - 1.300 |
| R-factor | 0.15567 |
| Rwork | 0.154 |
| R-free | 0.17935 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yli |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.078 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.210 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.110 | 0.650 |
| Number of reflections | 38076 | |
| <I/σ(I)> | 7 | 2.1 |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | AMMONIUM SULFATE, HEPES PH 7.5 |
